Flavoprotein disulphide oxidoreductases: protein engineering of glutathione reductase from Escherichia coli.

نویسندگان

  • R N Perham
  • A Berry
  • N S Scrutton
چکیده

Reduced glutathione (GSH) plays a crucial role in ensuring that other thiol groups remain reduced within the cell and is particularly important in the biosynthesis of DNA [for ;I review, see Holmgren ( 1985)l. Dihydrolipoamide dehydrogenase is an essential component of the 2-ox0 acid dehydrogenase multi-enzyme complexes (Reed, 1974; Perham, 1983) in which it acts to oxidize the dihydrolipoic acid residues of the lipoate acyltransferase components in an NAD + -dependent reaction: Lip(SH), + NAD' = Lips2 + NADH + H'

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Protein engineering of domains in flavoprotein disulphide oxidoreductases: contributions to folding and assembly.

Introduction The flavoprotein disulphide oxidoreductases constitute a growing family of homologous dimeric enzymes, with an important and diverse range of functions in vivo. Among its members are dihydrolipoyl dehydrogenase, glutathione reductase, mercuric reductase, thioredoxin reductase, trypanothione reductase and a related enzyme, NADPH peroxidase (for recent reviews of their structures and...

متن کامل

Glutathione-dependent activities of Trypanosoma cruzi p52 makes it a new member of the thiol:disulphide oxidoreductase family.

Trypanothione: glutathione disulphide thioltransferase of Try-panosoma cruzi (p52) is a key enzyme in the regulation of the intracellular thiol-disulphide redox balance by reducing glutathione disulphide. Here we show that p52, like other disulphide oxidoreductases possessing the CXXC active site motif, catalyses the reduction of low-molecular-mass disulphides (hydroxyethyl-disulphide) as well ...

متن کامل

Quantitative Comparison of Tree Pairs Resulted from Gene and Protein Phylogenetic Trees for Sulfite Reductase Flavoprotein Alpha-Component and 5S rRNA and Taxonomic Trees in Selected Bacterial Species

Introduction: FAD is the cofactor of FAD-FR protein family. Sulfite reductase flavoprotein alpha-component is one of the main enzymes of this family. Based on applications of this enzyme in biotechnology and industry, it was chosen as the subject of evolutionary studies in 19 specific species. Method: Gene and protein sequences of sulfite reductase flavoprotein alpha-component, 5S rRNA sequence...

متن کامل

Quantitative Comparison of Tree Pairs Resulted from Gene and Protein Phylogenetic Trees for Sulfite Reductase Flavoprotein Alpha-Component and 5S rRNA and Taxonomic Trees in Selected Bacterial Species

Introduction: FAD is the cofactor of FAD-FR protein family. Sulfite reductase flavoprotein alpha-component is one of the main enzymes of this family. Based on applications of this enzyme in biotechnology and industry, it was chosen as the subject of evolutionary studies in 19 specific species. Method: Gene and protein sequences of sulfite reductase flavoprotein alpha-component, 5S rRNA sequence...

متن کامل

A glutathione reductase from Escherichia coli.

Earlier studies had demonstrated that a flavoprotein capable of mediating a triphosphopyridine nucleotide (TPN)-linked reduction of Furacin (5-nitro-2-furaldehyde semicarbazone) was present in cell-free extracts of Furacin-susceptible strains of Escherichia coli, but was absent in extracts of resistant strains (I). Dr. Philip E. Hartman, who was then associated with our department, suggested to...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochemical Society transactions

دوره 16 2  شماره 

صفحات  -

تاریخ انتشار 1988